What will Fluorescence in situ hybridization (FISH) and confocal microscopy (LSCM) show in the detection of...
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Fluorescent in situ hybridization and laser scanning confocal microscopy are often used in research for detection of intracellular periodontal pathogens within human tooth.
rRNA FISH favors detection of growing bacteria. Therefore, the test result for Actinobacillus actinomycetemcomitans will show fluorescence.
Laser scanning confocal microscopy (LSCM) is used to determine whether fluorescent bacteria were intracellular. The test result will show that surface contour of the Actinobacillus actinomycetemcomitans with the surface rendered opaque in red.
You ARE cheating, aren't you? ; )
Edited to add... Yeah, I figured that's what it was, but I wanted to tease.
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